TABLE 6.
Characteristics of genes in 2Rh knocked down by RNAi
| Gene | RNAi line | Source | Non-knockdown progeny classes | dcr2 RNAi da-GAL4 progeny | Expected progeny ratio | Lethality | Stage |
|---|---|---|---|---|---|---|---|
| Nipped-B | 17704R-2 | NIG | 86 | 0 | 1:1 | Lethal | L |
| Nipped-A | 2905R-7 | NIG | 79 | 0 | 1:1 | Lethal | E |
| gusa | 8688 | VDRC | 62 | 0 | 1:1 | Lethal | E |
| CG17684 | 46596 | VDRC | 76 | 0 | 1:1 | Lethal | E |
| CG17665b | 46743 | VDRC | 41 | 0 | 1:1 | Lethal | P |
| CG17683 | 19180 | VDRC | 53 | 0 | 1:1 | Lethal | L, P |
| CG17883c | 30277 | VDRC | 89 | 59 | 1:1 | Semi-lethal | E, P |
| 17883R-4 | NIG | 98 | 48 | 1:1 | Semi-lethal | E, P | |
| Atf6 | 36504 | VDRC | 53 | 7 | 1:1 | Semi-lethal | L |
| CG2981 | 2981R-3 | NIG | 43 | 33 | 1:1 | Viable | NA |
| CG17486 | 17486R-1 | NIG | 60 | 17 | 3:1 | Viable | NA |
All progeny counts are for dcr2-enhanced RNAi knockdown with a daughterless (da)-GAL4 driver. Expected progeny ratios differ depending on the insertion location and viability of the RNAi construct. NIG, National Institute of Genetics; VDRC, Vienna Drosophila RNAi Center.
a
RNAi knockdown with gus was not enhanced with dcr2. Used da-GAL4 driver only.
b
Semi-lethal without dcr2 enhancement.
c
Weak semi-lethality present only in dcr2-enhanced knockdown. Viable without dcr2 enhancement.