Figure 2.—
The degradation of α1 requires multiple ubiquitin-conjugating enzymes. (A) Cycloheximide-chase analysis of α13HA turnover at 30° in wild-type, ubc13Δ, and mms2Δ cells. A representative experiment is shown at the top, and the quantitative results of multiple experiments are shown below. (B) Measurement of the α13HA degradation rate at 30° in wild-type, ubc4Δ, ubc5Δ, and ubc4Δ ubc5Δ cells. A representative experiment is shown at the top, and the quantitative results of multiple experiments are shown below.