Figure 3.—

Khd1 binds repetitive sequences in the FLO11 open reading frame. (A) Enrichment of FLO11 mRNA following immunoprecipitation from cells expressing either Khd1–TAP or untagged Khd1. (B) Khd1 target sequences from CLIP map to the FLO11 repetitive element. Histogram of read mappings overlaid on a dot plot highlighting the repetitive region of the FLO11 open reading frame from the ∑1278b genome (http://www.vivo.colostate.edu/molkit/dnadot/, window size = 11, mismatch limit = 1). (C) Enrichment of constructs following immunoprecipitation of Khd1–TAP. Enrichments expressed as the level of the transcript relative to ACT1 mRNA in the immunoprecipitate divided by the level of the transcript relative to ACT1 mRNA in the input. Values are average of four independent experiments. Error reported as standard deviation.