Figure 4.—

Khd1 represses translation through the FLO11 repeats. (A) Western blot analysis of GFP protein levels from constructs expressing GFP alone, or GFP fused to the FLO11 repetitive sequences. P_TEF–KHD1 is an overexpression construct. The only visible band detected from wild type, and the predominant band from khd1Δ, migrate at the same molecular weight as GFP alone, suggesting that translation initiated at the GFP start codon. The higher migrating band from khd1Δ may result from low levels of translation initiation inside the repetitive element that become visible after derepression. (B) GFP mRNA levels normalized to TUB1 mRNA levels for the strains shown in A. Values are average of four independent experiments. Error reported as standard deviation.