Figure 5.—

Khd1 regulates mother–daughter Flo11 expression. Fluorescence microscopy was used to visualize Flo11 protein expression from the FLO11∷HA allele. (A) Flo11 expression patterns in mother–daughter pairs. (B) Khd1 affects the frequency at which daughter cells express Flo11 protein. The chance that a mother cell gives rise to a daughter cell expressing Flo11 protein increases when KHD1 is deleted, independent of ASH1 and whether or not the mother cell expresses Flo11 protein. The frequency of a daughter cell expressing Flo11 protein being produced from a mother cell that expresses Flo11 protein was determined by dividing the number of these mother–daughter pairs by the total number of pairs in which the mother expresses Flo11 protein. The frequency of a daughter cell expressing Flo11 protein being produced from a mother cell that does not express Flo11 protein was determined by dividing the number of these mother–daughter pairs by the total number of pairs in which the mother does not express Flo11. Two hundred and fifty mother-daughter pairs were analyzed per genotype in each of nine separate trials. Error reported as standard deviation.