Figure 3.—

The cassette assay (“sufficiency”) results. (Top) Schematic of the cassette constructs in comparison with the wild-type genomic rescuing construct. (Bottom) Cassette constructs with the indicated CTD fragments were all tested in an egl-15(null) background. (A) Empty cassette (NH#1321, negative control). (B) E⁹⁹⁴-Q¹⁰²⁶ (the gray fragment in Figure 1A) is sufficient for mediating sex myoblast migration (NH#1322). (C) Y¹⁰⁰⁹ is required within the gray (E⁹⁹⁴-Q¹⁰²⁶) fragment to mediate sex myoblast migration (NH#1325). (D and E) Sex myoblast distribution for the wild-type (D) unique portion of the type IV isoform (NH#1348) or the same unique portion with the Y^1087F mutation (E; NH#1349).