Figure 2.—

PCP genes direct the accumulation of Mwh. (A–F) ptc-Gal4/UAS-fz wings. (A, C, and E) A 32-hr wing immunostained for Mwh. (B, D, and F) Adult wings. The same image is shown at two different magnifications. # indicates the middle of the ptc domain and * indicates the outside the domain. In the adult wings note that in the vicinity of the # the hairs tend to point away from the midline (point of highest Fz expression). Note also that the pattern of accumulation of Mwh in the pupal wings is altered in this region and no longer runs in the proximal distal direction (arrow). Outside of the ptc domain (*) hair polarity and Mwh accumulation are normal. (G–L) ptc-Gal4/UAS-pk wings. (I–L) Regions inside (#) and outside (*) the ptc domain. In this genotype hairs inside the domain tend to point toward the midline and once again hairs outside are not affected. The accumulation of Mwh is altered inside the domain (arrow) but not outside.