Figure 3.—

In and Mwh co-immunoprecipitate from wing disc cells. Co-immunoprecipitations of In and full-length Mwh in wild-type and fz mutant cells are shown. Wing discs from ptc-Gal4 UAS-mwh/UAS-HA-inturned (A), ptc-Gal4 UAS-mwh/UAS-HA-inturned; fz^P21/fz^P21 (B), or ptc-Gal4/UAS-HA-inturned (C). Wing disc extracts were incubated with anti-Mwh antibody (from rabbit). As a control, no anti-Mwh antibody was added to one sample. The protein complex was precipitated with protein A beads and then assayed by Western blotting using either anti-Mwh (from rat) or anti-In antibodies (mouse). Note that Mwh is able to pull down In and the interaction is independent of fz. In the absence of mwh expression In is not pulled down by anti-Mwh antibody (C). In some experiments we see a lower-molecular-weight band that presumably represents a breakdown product of In (C).