Figure 5.—

The In∷Mwh fusion protein accumulates at the proximal side of wing cells and in hairs. The In∷Mwh fusion protein localized in a zigzag pattern in pupal wing cells at and just before hair morphogenesis as detected by anti-In immunostaining (A–C) and anti-Mwh immunostaining (D–F). When anti-In immunostaining was used there was a null mutation in the endogenous in gene so that the only protein recognized by the anti-In antibody was the fusion protein. Similarly when anti-Mwh immunostaining was used there was a null mutation in the endogenous mwh gene. At later stages we found the fusion protein accumulated in hairs (G–L). In I the hair is stained using anti-Phosphotyrosine staining, which stains the surface of the hair. In L the hair is stained using phalloidin. Here the staining shows F-actin in the growing hair and also the actin rootlet that extends from the base of the hair toward the basal part of the cell. As is seen for the endogenous Mwh protein, accumulation is primarily in more proximal regions of the hair.