Figure 4.—

Clk mutants are not resistant to oxidative stress. (A and B) Sensitivity to oxidative stress of the Clk mutants was assessed by exposing adult worms to 240 μM juglone. Of the 10 Clk mutants assessed, only clk-2 worms showed increased resistance to oxidative stress compared to wild-type N2 worms. In contrast, clk-1, clk-3, clk-4, clk-7, clk-8, and clk-10 worms were more sensitive to oxidative stress than N2 worms. Sensitivity to oxidative stress during development was assessed by transferring eggs to plates containing either 0.2 mM (C) or 0.3 mM (D) paraquat. Under these conditions, only clk-2 worms were able to develop to a developmental stage further than wild-type worms. In contrast, clk-1, clk-3, clk-4, clk-5, clk-6, clk-7, and clk-10 worms all arrested at an earlier stage in development than N2 worms. Overall, Clk mutants are not more resistant to oxidative stress than wild-type worms either during development or during adulthood. In most cases, Clk mutants were more sensitive to oxidative stress than wild-type worms.