Figure 2.—

Mutants that exhibit abnormal Venus∷UNC-6 localization in neurons. (A) Wild type. (B) unc-51(e369) mutant. (C) unc-14(e57) mutant. (D) unc-104(e1265) mutant. (E) gh23 mutant. Arrows indicate cell bodies. White lines indicate axons. Anterior is to the right, lateral view. Bars, 5 μm. In the wild-type background, Venus∷UNC-6 showed a punctate distribution throughout the cell body and axon, except for the nucleus. In the unc-51(e369), unc-14(e57), unc-104(e1265), and gh23 mutants, Venus∷UNC-6 was accumulated in the neural cell bodies, and little Venus∷UNC-6 was in the axons. (F) Relative fluorescence intensities of the Venus∷UNC-6 in neuronal cell of unc-104(e1265) and gh23 mutants to wild-type worms. In each case, 20 neuronal cell bodies were examined and the results were averaged. Error bars show the standard error. *P < 0.01 (Student's t-test). In unc-51(e369) mutants and unc-14(e57) mutants, Venus∷UNC-6 is accumulated in some part of the neuronal cell body. Therefore, we could not compare the fluorescence intensity between these mutants.