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. Author manuscript; available in PMC: 2010 Jul 31.
Published in final edited form as: J Mol Biol. 2009 May 29;390(5):991–1006. doi: 10.1016/j.jmb.2009.05.072

Fig. 3.

Fig. 3

Free energy reaction profiles illustrate the six possible mechanisms used by proteins to mediate duplex formation by changing the energy levels of the substrate, transition state, product or some combination thereof. Evaluation of how the magnitude of the duplex kon, koff and Kd changes after addition of protein is used to discriminate between alternate mechanisms.