Table 1.

U3-18S duplex kinetic and thermodynamic parameters

	MINI-17-18S	U3 MINI-18S
	No protein Added	No protein Added	Imp3p and Imp4p added	Fold change+	ΔΔG (kcal/mol)
koff (18S)	(1.0 ± 0.1) × 10−4 s−1	ND	(2 ± 1) × 10−3 s−1	20-fold increase	1.7*
kon (18S)	(7 ± 1) × 105 M−1s−1	ND	(7 ± 1) × 105 M−1s−1	No change	~0
Kd (18S)	1.4 × 10−10 M**	ND	(4 ± 2) ×10−9 M

⁺Fold change of U3-18S with protein to MINI-17-18S without protein;

ND - not detectable;

^*ΔΔG = –RT(ln(k_{off (no protein added)} for MINI-17-18S duplex/k_{off (protein added)} for U3-18S duplex)) at 20 °C;

^**Calculated from k_off (18S) and k_on (18S) values.