Table 2.

U3-ETS duplex kinetic and thermodynamic parameters

	No protein added	Imp3p and Imp4p added	Fold change	ΔΔG (kcal/mol)
Kd (ETS)	(7 ± 2) × 10−7 M	(7 ± 3) × 10−9 M	~100 fold decrease	−2.7+
kon (ETS)	(6 ± 2) × 105 M−1s−1	(5 ± 1) × 105 M−1s−1	Not significant*	~0
koff (ETS)**	0.42 s−1	3.5 × 10−3 s−1

⁺ΔΔG = −RT(ln(K_{d (no protein added)}/K_{d (protein added)})) at 20 °C;

^*P > 0.05;

^**Calculated from k_on (ETS) and K_d (ETS) values.