Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
UMR106.01 osteoblasts respond to pericellular hypoxia. A: Immunofluorescent staining for hypoxia in UMR106.01 osteoblasts after 1- or 96-h treatment under 1% or 21% oxygen tension. Positive immunofluorescence indicates the presence of HypoxyProbe-1 adducts, which form at oxygen <100 mm Hg (1.4% oxygen). B: Immunoblotting for HIF-1α under 1% or 21% oxygen tension for 1–96 h. Abundant HIF-1α protein is detected in UMR106.01 cells after l–96h culture in 1% oxygen.
