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. Author manuscript; available in PMC: 2010 Jun 5.
Published in final edited form as: J Cell Biochem. 2010 May 15;110(2):457–467. doi: 10.1002/jcb.22559

Fig. 2.

Fig. 2

Hypoxic culture decreases Wnt antagonists sclerostin and Dkkl transcript and protein expression. A: Representative Western blot demonstrating decreased sclerostin expression after 48 or 96 h culture under 1% oxygen, β-actin is shown as a loading control. B: Quantitation of Sost transcript (solid line) or sclerostin protein (dashed line) in UMR106.01 cells cultured for 6–96 h under 1% or 21% oxygen. C: UMR106.01 cells under normoxia demonstrate greater expression of Sost than Dkk1, while sFrp1 is not detectable. D: Quantitation of Dkk1 transcript in UMR106.01 cells cultured for 6–96 h under 1% or 21% oxygen tension. Data are normalized to ActB transcript or β-actin protein, and are presented as mean expression at 1% relative to 21% oxygen at the same time point ±SEM; n = 3–5 per time point per condition. *P< 0.05, **P< 0.001.