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. Author manuscript; available in PMC: 2010 Jun 5.
Published in final edited form as: J Cell Biochem. 2010 May 15;110(2):457–467. doi: 10.1002/jcb.22559

Fig. 5.

Fig. 5

Hypoxia alters BMP signaling in osteoblastic cells. A: Sost expression is induced in UMR 106.01 in response to 24 h culture with BMP-2 (10, 250, or 500 ng/ml). B: Hypoxia does not alter transcription of BMP signaling pathway components Bmp2, Bmp4, or Bmp-RlA. C: Expression of BMP antagonists Gremlin and Noggin increases under hypoxia. D: pSmad 1/5/8 phosphorylation is attenuated in osteoblastic cells cultured under 1% oxygen compared to 21% oxygen. Bars represent transcript or Smad phosphorylation and are presented as mean expression at 1% relative to 21% oxygen at the same time point ±SEM. n =3–5 per time point per condition. *P<0.05 compared to control or vehicle-treated, time-matched samples; ***P<0.001 compared to control or vehicle-treated, time-matched samples.