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. Author manuscript; available in PMC: 2011 Apr 16.
Published in final edited form as: Cell. 2010 Apr 16;141(2):304–314. doi: 10.1016/j.cell.2010.02.035

Fig. 5. LIS1 enhances dynein-microtubule interaction under load.

Fig. 5

(A) Beads driven by single dynein motors were allowed to move along microtubules in a weak optical trap (black traces). At 100 nm bead displacement from the trap center, laser power was automatically increased (T=0, solid vertical line), subjecting dynein to a load of ~2 pN, significantly greater than the dynein stall force (“superstall” conditions). Subsequent bead positions are shown as red lines, and demonstrate prolonged persistence of DL bead (1:10 D:L) on microtubule and less so of DNL (1:9:10 D:N:L) bead. (B) Analysis of multiple superstall traces as in (A) revealed that average detachment times in both DL and DNL assays were significantly increased relative to D alone. Exponential decay constant ± SEM is shown in each subpanel.