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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Immunobiology. 2009 Oct 31;215(7):535–544. doi: 10.1016/j.imbio.2009.09.006

Figure 2. CRCL induces iNOS expression and NO production by macrophages.

Figure 2

(A) RAW264.7 cells were plated on slide chambers and exposed for 24 hrs to tumor-derived CRCL (25 μg/ml). Cells were then washed and stained for iNOS expression by IHC. Upper panels, 200× magnification, lower panels 400× magnification. (B) Western blot detection of iNOS expression in RAW264.7 or peritoneal macrophage treated with CRCL (25 μg/ml). (C) RAW264.7 cells or peritoneal macrophages were incubated for 12 hrs with CRCL (25 μg/ml) and nitrite concentration was evaluated in the culture supernatants. In all the experiments LPS (1 μg/ml)was used as a positive control. *, a significant difference when compared to untreated control cells (p < 0.05).