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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Immunobiology. 2009 Oct 31;215(7):535–544. doi: 10.1016/j.imbio.2009.09.006

Figure 3. CRCL triggers macrophage tumoricidal activity.

Figure 3

Peritoneal macrophages (50,000 cells per well) were cultured with B16 tumor cells (20,000 cells per well) and treated or not with tumor-derived CRCL (25 μg/ml) or with LPS (1 μg/ml). After 24 hrs cell viability was determined using a crystal violet toxicity assay. B16 cells alone (Medium) and B16 cells cultured with macrophages (+mφ) were used as negative controls. The data are representative of three independent experiments. *, a significant difference when compared to tumor cells culured alone (p < 0.05).