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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Cancer Prev Res (Phila). 2010 May 25;3(6):776–786. doi: 10.1158/1940-6207.CAPR-09-0189

Figure 6.

Figure 6

A. Differentially expressed genes in human bladder derived cells. Semi-quantitative RT-PCR of 25, 30, and 35 cycles was performed on four human bladder cell lines using primers for four of the genes identified from the UPII-SV40Tag mouse microarray experiment. The relative expression was determined by a ratio of each sample to the most intense sample for that gene, which was assigned a value of one. B. Immunohistochemical detection of differentially expressed genes in paraffin sections of superficial bladder TCC. Sections were probed with antibodies for the indicated proteins and detected colorimetrically using horseradish peroxidase-coupled secondary antibodies, except for the RacGAP1 20X and Hmmr 40X panels which were detected by fluorescently tagged secondary antibodies.