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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Cancer Invest. 2010 Jul;28(6):588–597. doi: 10.3109/07357900903286941

Figure 1. Ectopic p16 expression in human breast cancer MDA-MB-231 cells either by AdRSVp16 transduction or induction by Dox in a Tet-on regulatory system.

Figure 1

(a) and (b) MDA-MB-231 cells were grown in culture dish and transduced by recombinant adenovirus-expressing p16 (AdRSVp16) at multiplicity of infection (moi) of 200. After 72 hr, the cells were harvested and subjected to immunohistochemistry using primary antibody (mouse anti-human p16 antibody), followed by goat anti-mouse secondary antibody coupled with horseradish peroxidase. Shown are p16-immunostaining for control untreated cells (a), and cells transduced by AdRSVp16 (b). (c) and (d) MDA-MB-231 cells stably transfected by lentivirus-expressing inducible p16 protein under the control of Tet-on promoter (MDA-MB-231/Tet-on p16) were treated either without (c) or with (d) 1 μg/ml Dox for 72 hr. The cells were then IHC stained by anti-p16 antibody as described above. The dark brown color indicated p16 protein (b) and (d).