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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Cancer Invest. 2010 Jul;28(6):588–597. doi: 10.3109/07357900903286941

Figure 2. p16 downregulates VEGF expression at the protein and transcriptional levels.

Figure 2

(a) p16 expression decreased VEGF secretion. MDA-MB-231 cells were grown in medium containing charcoal-stripped serum. Cells were either untreated or transduced with control virus AdRSVlacZ or AdRSVp16 at moi of 200. The cell medium was collected 72 hr after viral transduction and subjected to VEGF determination by ELISA assay. (b) p16 neutralizes HIF-1α-mediated transactivation of VEGF gene promoter. pVEGF/Luc was cotransfected into MDA-MB-231 cells with control plasmid DNA or pAvsp16 DNA or pHIF-1α (pHIF-1alpha), or pAvsp16 plus pHIF-1α. (c) Tet-on inducible p16 expression inhibits the transactivation of VEGF gene promoter. MDA-MB-231/Tet-on p16 cells were treated with or without 1-μg/ml Dox and cotransfected with pVEGF/Luc and phRLuc-TK. All data represent the results from two independent experiments, each performed in triplicate. Error bars represent standard error (p < .05). Some error bars are too small to be shown in this scale.