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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Cancer Invest. 2010 Jul;28(6):588–597. doi: 10.3109/07357900903286941

Figure 3. p16 inhibited tumor-induced angiogenesis and neovessel formation in the air sac model.

Figure 3

The mouse in the air sac model was sacrificed on day 3 after chamber implantation and the implanted chamber was removed from the subcutaneous air fascia, a ring without filters was placed on the same site, and then photographed. The newly formed blood vessels were morphologically distinguishable from the pre-existing background vessels by their zigzagging characters (see representative arrows). Shown are undersurface images of sites from chambers containing PBS only as negative control (a), MDA-MB-231 cells (b), AdRSVlacZ-transduced MDA-MB-231 cells (c), AdRSVp16-transduced MDA-MB-231 cells (d), and MDA-MB-231 cells with 0.1 μg/ml neutralized anti-VEGF antibody (g). (e) and (f) are the magnified pictures of (c) and (d), respectively. Original magnifications: (a), (b), (c), (d), and (g) 7.5×; (e) and (f) 30×. Each group uses at least five mice and the representative photos were shown.