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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Cancer Invest. 2010 Jul;28(6):588–597. doi: 10.3109/07357900903286941

Figure 4. p16 forms a complex with HIF-1α.

Figure 4

MDA-MB-231 cells were transfected with HIF-1α expression vector (13) (lane 1–4) and transduced with either no virus (lane 1), control virus (lane 2), or AdRSVp16 (lanes 3 and 4) for 72 hr. Cell lysates were immunoprecipitated (IP) with anti-HIF-1α (lane 1–3) or preimmune IgG (as a negative control, lane 4); the immunoprecipitates (lane 1–4) were then analyzed by Western blot (WB) with anti-p16. The same blot was also immunoblotted with anti-HIF-1α. As a positive control for p16 protein, cell extracts overexpressing p16 protein (AdRSVp16-transduced cells) were used directly for Western blot by anti-p16 (lane 5).