Figure 5. Colocalization of p16 and HIF-1α protein.

MDA-MB-231 cells were cotransduced with Ad-HIF-1α and AdRSVlacZ at moi of 100 for each virus for 3 days. The cells were then fixed and proceeded for immunofluorescent staining using anti-p16 and anti-HIF-1α as primary antibodies as described in Materials and Methods section. The images were recorded by a confocal microscope: (a) p16 expression, (b) HIF-1α expression, and (c) merge of (a) and (b).