Figure 3. Release of FITC-SH from Au-NPs in tumour cylindroids.
a–f, Green fluorescence images and corresponding intensity profiles of cylindroids treated with nanoparticles. Fluorescence images were acquired after incubation with p-FITC-AuNP (a), n-FITC-AuNP (c) and FITC (e) for 11, 21, and 46hrs. Dotted circles indicate cylindroid edges and scale bar is 300mm. In b and d, fluorescence intensity profiles are shown at 11, 21, 38, and 46hrs. g and h, Change of average fluorescence intensities over time in the inner and outer regions of cylindroids (indicated with hashed bars in panel b and d) after incubation with p-FITC-AuNP (g) and n-FITC-AuNP (h). Errors are standard error of the mean (n=3). i, Blue fluorescence image of a cylindroid incubated with OPA for 18hrs. Scale bar is 200μm. j, green confocal fluorescence microscope image of the outer region of the cylindroid treated with p-FITC-AuNP. Scale bar is 20mm.
