Skip to main content
. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Cancer Prev Res (Phila). 2010 May 18;3(6):718–726. doi: 10.1158/1940-6207.CAPR-10-0048

Fig. 5.

Fig. 5

A, Reverse transcription-PCR for survivin and GAPDH (loading control) mRNA levels in MCF-7 and MDA-MB-231 cells treated for 16 h with DMSO (control) or the indicated concentrations of BITC. Number above band represents quantitation relative to corresponding DMSO-treated control. B, immunocytochemical analysis for survivin localization in MCF-7 cells following 16 h treatment with DMSO (control) or 5.0 µmol/L BITC. Staining for survivin, mitochondria, and nuclei is indicated by green, red and blue fluorescence, respectively. C, immunoblotting for survivin using isolated cytosolic and nuclear fractions prepared from MCF-7 cells following 16 h treatment with DMSO (control) or the indicated concentrations of BITC. The blots were stripped and reprobed with anti-PARP and anti-α-tubulin antibodies.