Fig. 6.
Immunoblotting for survivin using lysates from MCF-7 (A) and MDA-MB-231 cells (C) transiently transfected with a control nonspecific siRNA or a survivin-specific siRNA, and treated with DMSO (control) or 5.0 µmol/L M BITC for 24 h. Cytoplasmic histone-associated DNA fragmentation in MCF-7 (B) and MDA-MB-231 cells (D) transiently transfected with a control nonspecific siRNA or a survivin-specific siRNA, and treated with DMSO (control) or 5.0 µmol/L BITC for 24 h. Columns, mean (n= 3); bars, SD. Significantly different (P<0.05) compared with control nonspecific siRNA transfected cells treated with DMSO (a), and control nonspecific siRNA transfected cells treated with BITC (b) by one-way ANOVA followed by Bonferroni’s test. Results were consistent in two experiments, and representative data from one such experiment are shown.
