Fig. 3.

Histological section of normal primordial follicles in fresh tissue (3-A) and cultured for 6 days in control (3-B) and sequential medium (3-C); Degenerated primordial follicles cultured for 12 days in control (3-D) and constant (3-E) or sequential (3-C) medium, or degenerate primary follicle cultured for 18 days in constant medium (3-F), or degenerater secondary follicle cultured for 18 days in control (3-G) and sequential medium (3-H); Normal primary follicles cultured for 6 days in control (3-I), and constant (3-J) or sequential medium (3-K) ; Normal secondary follicle cultured for 18 days in constant medium (3-L) or 12 days in sequential medium (3-M); Antral-like follicle cultured for 18 days in control medium (3-N) or antral follicle cultured for 18 days in sequential medium (3-O). Note: Pycnotic nucleus (asterisk) and irregular granulosa cells (arrow); 3-A-M × 400, 3-N, O  × 100