Table 1.

Composition of sequential culture system

Day of culture	Composition
	EGF	FSH	GDF-9	bFGF
	ng/mL	mIU/mL	ng/mL	ng/mL
2	25	50	200	150
4	50	50	200	150
6	50	100	150	100
8	75	200	150	100
10	75	300	100	100
12	100	400	100	50
14	100	500	50	50
16	150	500	25	25
18	150	500	0	0