Table 3.
The apoptotic rates (number of apoptotic cells/total cells number) of follicles and stromal cells in non-cultured tissue and tissues at 6, 12, and 18 days after culture
| Culture medium | Apoptotic rate of follicles % | Apoptotic rate of stromal cells % (total stromal cell) | ||||
|---|---|---|---|---|---|---|
| Day of culture | Non-cultured tissue: 0 (0/23) | Non-cultured tissue: 5.7±1.1%(432) | ||||
| Control | Constant | Sequential | Control | Constant | Sequential | |
| 6 day | 7.5(5/67) | 4.1(3/73) | 3.4(2/61) | 13.3 ± 3.2a(571) | 9.4±2.5ab (600) | |
| 12 day | 11.3(6/53) | 6.3(4/63) | 6.7±2.0b(620) | |||
| 5.4(4/74) | 21.4 ± 3.7a*(631) | 12.7 ± 3.1b(628) | ||||
| 18 day | 18.8(8/48) | 7.1(5/70) | 9.5±1.6b(675) | |||
| 5.6(4/72) | 27.5 ± 5.4a *(606) | 17.1±1.8b*(710) | ||||
| 12.4±1.9b(691) | ||||||
*Significantly different from non-cultured tissue (P < 0.05)
a, bSignificantly different between culture media on the same day of culture (P < 0.05)