Fig. 1.

a Mammalian expression vector used for expression of the anti-Vaccinia and anti-RSV antibodies. CMV human CMV promoters, VH antibody heavy chain variable region, IgG1 constant genomic DNA encoding the human IgG1 constant region, IRES + DHFR ECMV internal ribosome entry sequence + mouse DHFR cDNA, SV40 poly A the SV40 polyadenylation sequence, bla and Amp ampicillin resistance gene, pUC ori pUC origin of replication, bGH poly A bovine growth hormone polyadenylation sequence, LC antibody light chain cDNA. b Comparison of specific productivities in selected pools of DG44 and ECHO cells. DG44 cells and ECHO cells were transfected with three different anti-Vaccinia virus antibody constructs (Ab numbers refer to the numbers used in Fig. 2). Specific productivities were measured in selected pools