Fig. 4.

Experimental setup of the compositional stability studies with compositions expressing the anti-RSV rpAb. Suspension ECHO cell clones expressing each of the six monoclonal anti-RSV antibodies were banked individually. Selected ECHO clones, one for each of the six anti-RSV antibodies, were thawed and mixed. The mixed compositions were immediately frozen as polyclonal master cell banks (pMCB). One ampoule of the pMCB was subsequently thawed, expanded, and frozen as polyclonal working cell bank (pWCB). pWCB cells were then thawed and seed-train expanded prior to a fed batch production run in bioreactors