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. Author manuscript; available in PMC: 2010 Jun 5.
Published in final edited form as: Lab Chip. 2010 Feb 9;10(9):1153–1159. doi: 10.1039/b921417h

Fig. 3.

Fig. 3

The denaturation profiles in three channels mimicking the sequencing of 6 bases. (A) The fluorescent signals were measured in three channels. (B) The negative derivatives of the fluorescence signals were taken. (C) The fit of the negative derivatives overlaps with the negative derivatives well. (D) By the order of the peaks of the component curves of the fit, the base sequence of the template DNA can be determined correctly. The peak positions are 46.0, 48.0, 48.0, 52.1, 53.5, 53.8 °C, respectively.