Table 1.

List of DNA sequences and melting temperatures. The bases where the template and the fluorescently labeled oligonucleotide probes hybridize to are underlined and the target region being sequenced is italicized

		Melting temperature(Tm)
Oligonucleotide	Sequence	Predicted	Solution	Surface
Target template	5′-Biotin-TEG-(Biotin-TEG)-TACAGACTTAGTGGGGTAAAACTAGCATGACTGACTTCGTACATGACTGATGGTCGATAC-3′
20merT_5Alx488	5′-Alexa488-CCATCAGTCATGTACGAAGT-3′	53.2	NDa	45.8
21merC_5Alx546	5′-Alexa546-CCATCAGTCATGTACGAAGTC-3′	55.1	54.1	48.0
22merA_5Alx647	5′-Alexa647-CCATCAGTCATGTACGAAGTCA-3′	57.3	54.3	49.8
24merT_5Alx488	5′-Alexa488-CCATCAGTCATGTACGAAGTCAGT-3′	60.5	56.9	53.0
25merC_5Alx546	5′-Alexa546-CCATCAGTCATGTACGAAGTCAGTC-3′	61.8	56.7	54.4
26merA_5Alx647	5′-Alexa647-CCATCAGTCATGTACGAAGTCAGTCA-3′	63.5	57.9	54.7
Alexa488 control	5′-Biotin-TEG-CCAT-(Biotin-TEG)-CAGTCATGTACGAAGTCAGT-Alexa488-3′
Alexa546 control	5′-Biotin-TEG-CCAT-(Biotin-TEG)-CAGTCATGTACGAAGTC-Alexa546-3′
Alexa647 control	5′-Biotin-TEG-CCAT-(Biotin-TEG)-CAGTCATGTACGAAGTCA-Alexa647-3′

^aNot determined.