Figure 2.

Cytokinesis defects as a result of DdCenB knock-out. (A) consists of images captured from time-lapse microscopy. The time of capture (minutes) for each frame is indicated in the lower right corners. The arrowheads point to the persistent cytoplasmic bridges observed in dividing cells. In (B) we see a wild-type cell dividing. Here the arrowhead points to a typical cleavage furrow. (C) is a dividing mutant cell stained with DA PI (DNA) and visualized by fluorescence/DIC microscopy. Arrows point to distorted nuclei in the furrow region. (D) shows a mutant cell, with a long cytoplasmic bridge, stained with DAPI. The arrows point to higher magnifications of the respective areas, and highlight the presence of DNA in the cytoplasmic bridge. D, fluorescence; D’, phase contrast. Bars in all cases correspond to 5 µm.