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. 2010 May-Jun;2(3):275–284. doi: 10.4161/mabs.2.3.11676

Figure 1.

Figure 1

Expression and detection of nucleoprotein N. (A) of recombinant Trx (1) and Trx-N fusion protein (2) in E. coli cells as detected by Coomassie staining of Ni-NTA column purified bacterial extracts, or by anti-RVFV rabbit (3) or sheep (4) hyperinmune sera. (B) Inmunoprecipitation of BHK21 cell supernatants (S) and cell lysates (CL) with a rabbit anti-RVFV polyclonal serum. (1) MP12 infected cells, (2) mock infected cells, (3) pCMV transfected cells and (4) pCMV-N transfected cell extracts. (C) Inmunoprecipitation of supernatant (S) and cell lysates (CL) of MP12 infected BHK21 cells using serum from mice immunized with pCMV-N (2 and 3) or sheep anti-RVFV hyperinmune serum (1). Mr: relative molecular mass in kilodaltons.