Skip to main content
. 2010 May-Jun;2(3):275–284. doi: 10.4161/mabs.2.3.11676

Figure 6.

Figure 6

(A) Performance of mAb F1D11 and A9F12 as capture antibodies in ELISA. Positive (experimentally vaccinated sheep, shaded bars and rabbit anti-RVFV hyperinmune serum, open bar) and negative sheep sera (1/120 dilution) were tested in ELISA for their ability to bind to mAb captured viral antigen from infected cell culture supernatants. An anti-sheep HRP-conjugated (diluted to 1/6,000) was used for detection of the inmunocomplexes. (B) Competitive using either purified A9F12 mAb or rabbit anti-RVFV serum as capture antibodies. experimental sera from vaccinated sheep were used to compete the binding of A9F12-HRP labelled antibody.