Table 1.
Characterization of mAbs anti-RVFV nucleoprotein
Immunogen | Name | Rec ELISAa | Capture ELISAa | WBb | ICCb | IIFb | Ig isotype |
DNA only | D7D8 | ⩽3 | 27 | − | + | + | IgG2a/K |
DNA only | D9D11 | 2187 | ⩾2187 | + | + | + | IgG2a/K |
DNA only | D7E8 | − | 27 | − | + | + | IgG2a/K |
DNA only | A9F12 | 2187 | ⩾2187 | + | + | + | IgG2a/K |
DNA + protein | F1D11 | 27 | 81 | + | + | + | IgG2a/K |
DNA + protein | F1B10 | 243 | 729 | + | + | + | IgG2a/K |
mean reciprocal dilution of three sample replicates which generates an OD450 nm reading of 1.5.
cells infected with RVFV MP12 strain. WB, western blot; ICC, immunocytochemistry; IIF, indirect immunofluorescence.