Figure 2.

Heatmap illustrating fold change (log2) in expression of cGMP-induced genes in the Arabidopsis GA-deficient mutants ga1-3 and penta. In order to reveal the transcriptional relationship between cGMP-induced genes and GA, expression of the top 59 cGMP upregulated genes was investigated in a publicly available microarray data set (Accession no: E-MEXP-849) containing the GA-deficient mutants ga1-3 and penta (a gal-3 della mutant lacking all four DELLA proteins).³⁰ Gene expression M values [log2(mutant/wildtype)] were determined for seed and flower tissues in both mutants as previously described. The heatmap indicates clusters of up-and downregulated GA-responsive genes, most noticeably in the gal-3 mutant with evidence of tissue-specific expression between seeds and leaves.