Table 1.
Summary of selected gene families associated with suberin and cutin biosynthesis—known members and potential new candidates
| AGI ID | Gene name | Described function | Cell type enriched11 | SLR R SCR (endodermis) | SLR S CER (epidermis) |
| 3-ketoacyl CoA synthase | |||||
| At1g01120 | KCS1 | cutin18 | R endo/S epi | 2.73 | 2.32 |
| At1g04220 | KCS2 (DAISY) | suberin,19 cutin20 | n.s. | 0.97 | 0.88 |
| At1g07720 | KCS3 | potential new target | S epi | 0.06 | 2.68 |
| At1g25450 | KCS5 | potential new target | S epi | −0.16 | 2.47 |
| At1g68530 | KCS6 (CUT1, CER6) | cutin21 | n.s. | 0.07 | 1.75 |
| At2g16280 | KCS9 | potential new target | S epi | 0.85 | 2.46 |
| At2g26250 | KCS10 (FDH) | cutin22 | R endo | 2.29 | 2.27 |
| At2g46720 | KCS13 (HIC) | potential new target | S epi | 0.03 | 1.79 |
| At4g34250 | KCS16 | potential new target | S epi | 0.27 | 2.15 |
| At4g34510 | KCS17 | potential new target | R endo | 1.68 | 0.08 |
| At5g04530 | KCS19 | potential new target | S epi | 0.04 | 2.98 |
| At5g43760 | KCS20 | cutin20 | n.s. | 1.19 | 1.93 |
| ketoacyl CoA reductase | |||||
| At1g67730 | KCR1 | cutin, suberin24 | R endo | 1.71 | 1.58 |
| long-chain acyl-CoA synthetases | |||||
| At2g47240 | LACS1 (CER8) | cutin35 | S epi | 0.11 | 3.36 |
| t1g49430 | LACS2 | utin36 | endo | .34 | .02 |
| At1g64400 | LACS3 | potential new target | R endo | 1.55 | 1.81 |
| fatty acid omega-hydroxylase | |||||
| At5g58860 | CYP86A1 (HORST) | suberin26 | R endo | 2.33 | −0.01 |
| At4g00360 | CYP86A2 (ATT1) | cutin27 | R endo/S epi | 2.68 | 2.62 |
| At1g01600 | CYP86A4 | cutin28 | S epi | 1.53 | 2.37 |
| At2g45970 | CYP86A8 (LCR) | cutin29 | R endo/S epi | 1.97 | 2.65 |
| At5g23190 | CYP86B1 | suberin30 | R endo | 2.71 | −0.02 |
| At5g08250 | CYP86B2 | suberin30 | R endo | 1.90 | 0.02 |
| At5g63450 | CYP94B1 | potential new target | S epi | 0.79 | 2.04 |
| other CYP450 enzymes | |||||
| At4g15330 | CYP705A1 | potential new target | R endo | 1.94 | 0.34 |
| At4g22710 | CYP706A2 | potential new target | S epi | −0.52 | 2.10 |
| At2g34490 | CYP710A2 | potential new target | S epi | 0.15 | 2.52 |
| At5g24900 | CYP714A2 | potential new target | R endo | 1.24 | −0.12 |
| At3g53280 | CYP71B5 | potential new target | S epi | 0.08 | 2.24 |
| At2g26710 | CYP734A1 | potential new target | S epi | −0.19 | 2.54 |
| At3g10570 | CYP77A6 | cutin28 | S epi | 0.07 | 1.82 |
| At1g11600 | CYP77B1 | potential new target | S epi | −0.02 | 1.92 |
| At5g09970 | CYP78A7 | potential new target | S epi | 0.13 | 1.31 |
| At5g52320 | CYP96A4 | potential new target | S epi | −0.33 | 2.11 |
| alcohol dehydrogenase | |||||
| At1g72970 | HTH | cutin32 | S epi | 0.37 | 2.64 |
| aldehyde dehydrogenase | |||||
| At4g36250 | ALDH | potential new target | S epi | −0.15 | 2.47 |
| glycerol-acyl-transferase | |||||
| At4g01950 | GPAT3 | potential new target | S epi | 0.32 | 2.12 |
| At1g01610 | GPAT4 | cutin25 | R endo | 3.48 | 2.88 |
| At3g11430 | GPAT5 | suberin33 | R endo | 2.80 | −0.18 |
| At2g38110 | GPAT6 | cutin28 | R endo | 3.01 | 0.41 |
| At4g00400 | GPAT8 | cutin25 | R endo/S epi | 2.37 | 2.83 |
| feruloyl-acyl-transferase | |||||
| At5g41040 | ASFT (ACT) | suberin31, 34 | R endo | 3.43 | 0.14 |
SLR, mean signal log ratio of pairwise comparisons of a specific cell type (here: pSCR or pCER 5) to all other non-overlapping cell types of the same organ;11 n.s., not significant.