Figure 5. Selected pathways leading to respiratory deficiency in C. glabrata contribute to increased susceptibility to PDT.
To better reveal differences in sensitivity to PDT among the strains, organisms were adjusted to 107 cells/ml, incubated with a range of concentrations (0.5 – 2.0 μg/ml) of TMP-1363 for 10 min and irradiated with a fluence of 2.4 J/cm2 broadband visible light (Irradiated). Organisms treated with TMP-1363 but shielded from light were used as controls (Shielded). Organism killing was determined by spotting 2 μl of ten-fold serial dilutions of treated cell suspensions on YPDextrose plates, followed by incubation at 37°C for 24 h. Representative results are shown. Abbreviations: RC (respiratory-competent); RD (respiratory-deficient).