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. Author manuscript; available in PMC: 2010 Jun 5.
Published in final edited form as: Biochemistry. 2003 Sep 9;42(35):10498–10505. doi: 10.1021/bi0300635

Figure 3.

Figure 3

Human PMNs can secrete nociceptin upon degranulation. (A) PMNs [10 × 106/mL] were stimulated as indicated and then processed for CD63 and CD66B surface marker determination by FACS using PE- and FITC-labeled specific antibodies. CD 63 and CD 66B are markers of azurophilic and specific granules, respectively (fMLP: 1 μM; PMA: 100 nM; CB: 10 μM). (B) Cell-free supernatants of PMNs stimulated in panel A were assayed for the presence of nociceptin by specific ELISA. Results presented are from one experiment representative of three independent experiments performed with PMNs from different donors.