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Restriction fragment length polymorphism and Southern blot analysis of recombinant MDV BAC. DNA of pRB-1B and mutant BAC derived thereof was prepared, digested with PstI, and separated on a 0.8% agarose gel together with 1-Kb marker (M, Promega) and a Dig-labeled marker (M-D). Southern blot analysis was performed using PCR-generated digoxigenin-labeled probes using oligonucleotide primers specific for the kanamycin cassette (middle panel) or the PchTR sequence (right panel).
