Table I.
Primers used in this study.
| Primers | Sequence 5′→3′ | |
|---|---|---|
| PchTR | AAAAAAGCTTCAAGAGATCGGCGTTGCTTTC | Underlined HindIII site |
| PchTR-R | AAAAGAATTCGCCTCCACCCGCCACGCGTG | Underlined EcoRI site |
| pEPchTRpromIN-F | AAAACTGCAGCTAGCTTGCTGGCGGTGTCCGGAGCAAA TCCGCATCTCGAACTGTAGGGATAACAGGGTAATCGATTT | Underlined PstI site |
| pEPchTRpromIN-R | AAATCTGCAGGCCAGTGTTACAACCAATTAACC | Underlined PstI site |
| PvTRexch-Forw | CCCCAAAAACCTCTCGCGGCCGACGCGCCCCCAGGGATGTAATTACGTCCCTCCCCCCTAGGGGGCAGCAGGCCAAGAGATCGGCGTTGCTTTC | |
| PvTRexch-Rev | G ACACAGCGGAGCCTTCCACCCGCCACGTGTGCCTTCCACCCGCCACGCGTG | |
| Resc-PvTR-F | AAGCTTGACAGGCAGTTGTACACCTG | Underlined HindIII |
| Resc-PvTR-R | GAATTCCTGAAAGTCAGCGAGTAAAC | Underlined EcoRI site |
| pEP-PvTR-F | GGATCCGATCCCGCAGACCCCGGCCCACAGGAAGGGGCGGGGCACGTGCAGGATGACGACGATAAGTAGGGATAAC | |
| pEP-PvTR-R | GGAT CCGGGTAATGCCAGTGTTACAACCA | |
| EPrescPvTR-F | CCAAAAACCTCTCGCGGCCGACAGGCAGTTGTACAC CTG | |
| Kan-F | TAGGGATAACAGGGTAATCGATTTATTC | |
| Kan-R | GCCAGTGTTACA ACCAATTAACC | |
| SB-PchTR-forw | CAAGAGATCGGCGTTGCTTT | |
| SB-PchTR-rev | CAAGCTAG CTGCAGTCTCAG | |
| qRT-PCRvTR-Forw | CCCTAGCCCGCTGAAAGTC | |
| qRT-PCRvTR-Rev | CCTAATCGGAGGTATT GATGGTACTG | |
| vTR-probe | Fam-CCCTCCGCCCGCTGTTTACTCG-BHQ-1 |