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. Author manuscript; available in PMC: 2010 Jun 7.
Published in final edited form as: DNA Repair (Amst). 2006 Mar 29;5(5):618–628. doi: 10.1016/j.dnarep.2006.02.005

Figure 2.

Figure 2

Esc4 co-immunoprecipitates with the Rad55–57 heterodimer. Protein extracts were prepared from control and MMS-treated cells and subjected to immunoprecipitation with anti-Rad57 antibody (lanes 1–4). Experiments lacking the anti-Rad57 antibody were also performed (lanes 5 and 6). Immunoprecipitates were analyzed for the presence of Rad55 and Esc4 with anti-Rad55 and anti-Myc antibodies, respectively. In cells lacking myc-tagged Esc4, only Rad55 is detected (lanes 1 and 2), consistent with the presence of the Rad55–57 heterodimer. In ESC4-13myc cells, both Rad55 and Esc4-13myc are detected (compare lanes 3 and 4 with lanes 5 and 6).