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. Author manuscript; available in PMC: 2010 Jun 7.
Published in final edited form as: DNA Repair (Amst). 2006 Mar 29;5(5):618–628. doi: 10.1016/j.dnarep.2006.02.005

Figure 7.

Figure 7

Esc4 forms S-phase specific foci in response to MMS. (A) Esc4-GFP(S65T) exhibits three types of nuclear localization: diffuse (upper), 1–2 foci at the edge of the nucleus (middle), or ≥3 distinct foci (lower). (B) Treatment of wild-type cells with MMS increases the percentage of budded cells that contain ≥3 foci. (C) Nuclear foci are also observed in S-phase cells in response to treatment with 100 mM HU for 1 hr. (D) Foci observed in the nucleus of undamaged S-phase cells may be localized to the rDNA. rDNA is visualized with Nop1-RFP (red); nucleus is stained with DAPI (blue). In cells lacking either MRC1 (E) or TOF1 (F), a significant number of budded cells contain foci in the absence of MMS.