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. Author manuscript; available in PMC: 2011 Jun 9.
Published in final edited form as: Cell Metab. 2010 Jun 9;11(6):467–478. doi: 10.1016/j.cmet.2010.04.005

Figure 6. Metabolic effects of liver Atg7 restoration in obese mice.

Figure 6

A. Gross anatomical views of representative livers of ob/ob mice expressing Atg7 (Ad-Atg7) or control vector (Ad-GFP). B. Representative images of H&E staining (40×) in ob/ob mice liver expressing Atg7 or control vector. Triglyceride (Tg) content of the liver (C), serum insulin level (D) were measured in ob/ob mice expressing Atg7 (n=8) or control vector (n=8). Data are shown as mean±SEM. Asterisk indicates statistical significance determined by student’s-t test (*p<0.05). E. Glucose tolerance test (GTT) performed in ob/ob mice following injection of Adeno-GFP (Ad-GFP, n=8) or Adeno-Atg7 (Ad-Atg7, n=8). F. Insulin tolerance test in ob/ob mice following injection of Ad-GFP or Ad-Atg7. All data are presented as mean±SEM, with statistical analysis performed by repeated measures two-way ANOVA (* indicates p<0.05). Hyperinsulinaemic–euglycaemic clamp studies were performed in ob/ob mice transduced with Ad-GFP (n = 7) or Ad-Atg7 (n = 6). Basal and clamp hepatic glucose production (HGP) (G–H), glucose infusion rate (GIR) (I) and glucose uptake in gastrocnemius muscle (J) were analyzed. Data are shown as the mean±SEM, *P < 0.05.