Figure 2.

Illustration of key “wet-lab” cardiac tissue processing stages. (a) Low resolution image of a black wax-embedded rat heart ready for sectioning, showing left ventricle [LV], right ventricle [RV] and aorta [A]. (b) Corresponding Trichrome stained montage image of an entire long-section, taken from the wax block shown in (a). (c) High-resolution fragment of the same image, obtained using ×63 objective, revealing sub-cellular detail such as sarcomeres [S], nuclei [N] and interstitial spaces [IS]. (d) Long-axis MRI section, from the same heart, before processing for co-alignment of histology and MRI data (see Section e, below).